International Journal of Stem Cells : eISSN 2005-5447

Fig. 2.

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Fig. 2. Effect of Noni (200 μg/ml) on rat bone marrow derived stem cells after 5 days and before inducing with osteogenesis media. Morphology of cells (A) without and with (B) Noni on the fifth day (40× objectives). (C) Transcriptional analysis for the expression of mesenchymal stem cell marker genes CD 44 and CD 90 generated from 5 independent experiments that were normalized with β actin. (D) Growth curve of rat BMSC through 6 days. At day 1, 2, 3, 4, 5, 6 first passage BMSCs in DMEM with or without noni were plated in each well of a 96-well plate; cell number was then evaluated at different times via Thiazolyl blue (MTT) staining. Noni at a concentration of 200 μg/ml showed a significant increase in BMSC proliferation from 2nd through 5th day. MTT results are reported as percentages (BMSC-bone marrow derived mesenchymal stem cells).
International Journal of Stem Cells 2016;9:221-9
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