International Journal of Stem Cells : eISSN 2005-5447

Table. 1.

Table. 1.

PCR conditions

StepTemperature Time 
Initial Denaturation 95°C5 min
Cycle
 Denaturation95°C30 s
 Annealing*Determined according to target 30 s
 Extension72°C30 s
Final Extension72°C5 min

Annealing temperatures: CD44: 53°C, CD90: 54°C, CD105: 55°C, Beta-actin: 53°C.

International Journal of Stem Cells 2017;10:184-92 https://doi.org/10.15283/ijsc17028
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