International Journal of Stem Cells : eISSN 2005-5447

Fig. 3.

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Fig. 3. Effect of NAM treatment on osteogenesis. (A) BMSCs cultured in the absence or presence of NAM for PD 4, 6, 8, or 10 (or PD13 in NAM (+) culture) were plated in 24-well plates and induced to osteogenesis in OIM containing NAM for 12 days. Cells were stained with Alizarin Red S and photographed under a microscope. (B, C) BMSCs cultured in MSCGM in the absence or presence of 5 mM NAM for PD 6 or 8 were plated in 24-well plates and induced to osteogenesis in OIM with or without 5 mM NAM for 12 days. Cells in each wells were stained with Alizarin Red S and photographed (B) or extracted with acid and neutralized, and applied for quantitation through observance at 405 nm in spectrometry. The significance of the difference between (−) and (+) NAM in MSGCM was determined by ANOVA test (Dunnett’s test) (*p<0.1; **p<0.01).
International Journal of Stem Cells 2018;11:13-25 https://doi.org/10.15283/ijsc18033
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