International Journal of Stem Cells : eISSN 2005-5447

Fig. 4.

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Fig. 4. Effects of NAM treatment on adipogenesis and chondrogenesis. (A) BMSCs cultured in the absence or presence of 5 mM NAM for PD 8 were plated in 24-well plates and induced to adipogenesis for 9 days in AIM without or with NAM. Cells were stained with 0.2% Oil red O and photographed under a microscope. (B) For quantification of lipid accumulation, BMSCs were treated as in (A) (except for incubation in 6-well plates), induced to adipogenesis, and stained with Oil Red O. Oil droplets were dissolved in isopropanol and absorbance at 518 nm was determined using a spectrophotometer. Two biological repeats from two independent experiment were quantitated and mean values were plotted. The significance of the difference between (−) and (+) NAM in MSGCM was determined by ANOVA test (Dunnett’s test) (*p<0.1; **p<0.01). (C) BMSCs cultured in MSCGM in the absence or presence of 5 mM NAM for PD 8 were plated in 24-well plates and induced to chondrogenesis for 15 days in CIM without NAM or containing 5 mM NAM. Cells were stained with Alician Blue solution and the spheroids were visualized under light microscopy and photographed.
International Journal of Stem Cells 2018;11:13-25 https://doi.org/10.15283/ijsc18033
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