International Journal of Stem Cells : eISSN 2005-5447

Fig. 4.

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Fig. 4. EAE-MSCs differentially regulate neural cell development and oligodendrocyte formation. (A) Top pathways (ranked by p-value) associated with the DEGs found between peak EAE-MSCs and naïve MSCs (left) or chronic EAE-MSCs and naïve MSCs (right). The number in parenthesis next to the pathway identifies statistical ranking assigned by MetaCore. Notable immune and neural cell pathways have been highlighted in green and red respectively. (B) Heatmap showing expression changes between peak EAE-MSCs and naïve MSCs (left) or chronic EAE-MSCs and naïve MSCs (right) for genes important in regulating neural cell development. (C) Neural cell cultures treated with conditioned medium (CM) from naïve MSCs had significantly higher number of MBP+ oligodendrocytes compared to control cultures treated with unconditioned medium, whereas cultures treated with EAE-MSC CM had a higher number of GFAP+ astrocytes relative to controls and they appear more reactive (insert). Scale bar=30 μm, data shown in graph=mean+SEM, **p<0.01, One-way ANOVA.
International Journal of Stem Cells 2018;11:48-60 https://doi.org/10.15283/ijsc17062
© 2018 International Journal of Stem Cells