International Journal of Stem Cells : eISSN 2005-5447

Fig. 4.

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Fig. 4. Multipotent differentiation ability of SHED cultured in MSCGM-CD or FBS-containing medium. (A) Phase contrast images of SHED derived osteoblast-like cells, as shown by calcified nodules formation which appeared during alizarin red staining. (B) Alcian blue staining showed proteoglycans in chondrocyte cultures differentiated from SHED grown in both media. (C) After induction with spiral ganglion neurons induction medium for 14 days. The expression of the mature neuronal marker Tuj1 (green) and spiral ganglion neurons markers TrkB (red) is shown. Cell nuclei were counterstained with DAPI (blue). Magnification: A, 40×; B, 100×; C, Scale bar=20 μm. SHED, stem cell from human exfoliated deciduous teeth, Tuj1, β-tubulin III, TrkB, tropomyosin receptor kinase B, DAPI, 4′,6-diamidino-2-phenylindole.
International Journal of Stem Cells 2018;11:96-104
© 2018 International Journal of Stem Cells