International Journal of Stem Cells : eISSN 2005-5447

Fig. 1.

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Fig. 1. Expression and colocalization of stem cell markers in the leaflet (A~J). IHC staining showed the presence of c-Kit+ (A; arrows) and rare Oct-4+ cells (B; arrows) within VECs. Expression of α-SMA was observed in a subset of multinucleated cells (C; arrows) below the endothelial surface of the ventricular side. The positive staining for PCNA showed the proliferation potential of VECs and VICs (D; arrows). The expression of pluripotent stem cell markers SSEA-1 (E), SSEA-4 (F), Tra-1-60 (G), and Tra-1-81 (H) was not detected in the representative tissue sections. Co-expression of c-Kit (red) and e-Nos (green) in VECs (I; arrows) was confirmed by immunofluorescent staining. In addition, a subset of c-Kit+ cells (red) was found in the subendothelial region directly adjacent to the α-SMA+ multinucleated cells (J; green). DAPI (blue) was used for counterstaining of the nuclei.
International Journal of Stem Cells 2018;11:121-30
© 2018 International Journal of Stem Cells