International Journal of Stem Cells : eISSN 2005-5447

Fig. 3.

Download original image
Fig. 3. Characterization of different types of multinucleated cells in a culture of the leaflet (A~D). Expression of c-Kit was detected in the majority of non-adherent mononuclear and multinuclear cells (A, B; red). Versican was expressed by multinucleated cells and found in the inner cavity of blastula-like colonies, suggesting a potential role for versican in cell aggregation and tissue regeneration (A, B; green arrows). Myofibroblast characteristics of the adherent cells surrounding the niche area were identified by expression of α-SMA (C; green). Expression of pluripotent markers Nanog and Oct-4 were detected in the newly formed niche areas before progressively decreasing during development of multinucleated colonies (C, D; red). Expression of the endothelial cell marker e-Nos in mature colonies showed spontaneous differentiation of pluripotent stem cells into endothelial cells (D; green).
International Journal of Stem Cells 2018;11:121-30
© 2018 International Journal of Stem Cells