International Journal of Stem Cells : eISSN 2005-5447

Fig. 2.

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Fig. 2. Characterisation of iPSC colonies. (A) Representative phase images of iPSC derived from fibroblasts transfected RF and cells transfected with RF+shpMbd3. Morphologically they appear to be similar. Immunostaining with pluripotency markers (green) and counter staining with DAPI (blue) of iPSC derived from fibroblasts transfected with RF only and those transfected with RF+shpMbd3 with TRA-1-81 and TRA-1-60. Scale bar is 200μm. (B) Q-PCR showed expression of pluripotency genes (Oct-4 and Nanog respectively) in cells treated with RF and RF+shpMbd3. All results were normalised to GAPDH. (n=3 Error bars±S.E.M). (C) Q-PCR showed up- regulation of lineage specific genes: Cdx2 (mesoderm), Pax6 (ectoderm) and MixI (endoderm) in cells treated with RF embryoid bodies relative to respective iPSC line (n=3 Error bars± S.E.M).
International Journal of Stem Cells 2018;11:235-41 https://doi.org/10.15283/ijsc18036
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