International Journal of Stem Cells : eISSN 2005-5447

Fig. 1.

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Fig. 1. The pattern of Tcfs and Lef1 expression in ES cells. (A) A6P10 ES cells were differentiated in the absence of LIF (left), in N2B27 medium without LIF (middle), or via EB formation without LIF (right). The differentiated ES cells were harvested on the days indicated. The amount of Tcfs and Lef1 transcripts was measured by RT-PCR analysis. The bands for Tcfs and Lef1 represent wild-type transcripts including β-catenin-binding domain. Self-renewal marker (Nanog), neural precursor markers (Sox1 and Fgf5) for N2B27 differentiation and mesodermal markers (Brachyury, Nkx2.5, αMHC) for EB differentiation were used to confirm proper differentiation; 18s rRNA was used as a loading control. (B) The endogenous protein levels of Tcfs/Lef1 were detected by western blotting. β-actin was used as a loading control.
International Journal of Stem Cells 2020;13:192-201 https://doi.org/10.15283/ijsc20044
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