International Journal of Stem Cells : eISSN 2005-5447

Fig. 2.

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Fig. 2. The effect of ectopic expression of Tcfs and Lef1 after LIF depletion. (A) A schematic diagram of Tcfs and Lef1 constructs (FL, full length; and DN, dominant negative). (B, C) A6P10 ES cells were transfected with control vector, Tcf1 (FL or DN), Lef1 (FL or DN), Tcf3 (FL or DN), or Tcf4 (FL or DN) and then selected using Zeocin. Selected ES cells were stained with alkaline phosphatase (AP), a marker of self-renewal, on the days indicated after LIF removal.
International Journal of Stem Cells 2020;13:192-201 https://doi.org/10.15283/ijsc20044
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