Fig. 1. p21 is involved in inhibitory effects of RUNX1b on the mesoderm-hemogenesis transition. D0-induced RUNX1b/hESC co-cultures with AGM-S3 cells at D4, (A) Cell-cycle analyses revealed that the proportion of cells in S phase dropped significantly when RUNX1b was overexpressed from D0, and that these inhibitory effects could be partially rescued by RepSox. (B, C) qRT-PCR and western blotting analysis showed that p21 was upregulated when DOX was added from D0, and that this effect could be counteracted by 0.33 μM RepSox. Grayscale scanning analyses were performed using Gel-Pro Analyzer 4.
© 2020 International Journal of Stem Cells