International Journal of Stem Cells : eISSN 2005-5447

Fig. 1.

Download original image
Fig. 1. p21 is involved in inhibitory effects of RUNX1b on the mesoderm-hemogenesis transition. D0-induced RUNX1b/hESC co-cultures with AGM-S3 cells at D4, (A) Cell-cycle analyses revealed that the proportion of cells in S phase dropped significantly when RUNX1b was overexpressed from D0, and that these inhibitory effects could be partially rescued by RepSox. (B, C) qRT-PCR and western blotting analysis showed that p21 was upregulated when DOX was added from D0, and that this effect could be counteracted by 0.33 μM RepSox. Grayscale scanning analyses were performed using Gel-Pro Analyzer 4.
International Journal of Stem Cells 2020;13:202-11
© 2020 International Journal of Stem Cells