International Journal of Stem Cells : eISSN 2005-5447

Fig. 2.

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Fig. 2. Construction and confirmation of inducible p21/hESC lines. (A) Schematic representation of the PiggyBac constructs used to inducibly express p21. TRE, tet-on regulation element; CMV mini, cytomegalovirus minimum promoter; T2A, Thosea asigna virus 2A peptide. (B) After p21/hESCs were induced for 48 h, the cells were imaged by fluorescence microscopy to observe co-expression of GFP. (C, D) qRT-PCR and western blotting were used to confirm that inducible expression of p21 was highly stringent and efficient at the transcriptional and protein levels. (E) Pluripotency of p21/hESCs (non-induced or induced) was confirmed by western blotting for SOX2, OCT4, and NANOG.
International Journal of Stem Cells 2020;13:202-11
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