International Journal of Stem Cells : eISSN 2005-5447

Fig. 4.

Download original image
Fig. 4. Colony assays confirmed the inhibitory effects of p21 on hematopoiesis. D0-induced or non-induced co-cultures of p21/hESCs with AGM-S3 (about 3×104 cells) at D12 were subjected to colony culture assays to assess their hematopoietic potentials. (A) The number of each type of colony derived from 3×104 co-cultured cells. p<0.05 was considered significant. (B) Typical morphologies of CFU-Mix (a), CFU-E (b), BFU-E (c), and CFU-GM (d) colonies. Scale bar, 100 μm. MGG staining of cells in BFU-E colonies (e). Scale bar, 10 μm. The hematopoietic potential of p21-induced co-cultures dropped significantly.
International Journal of Stem Cells 2020;13:202-11
© 2020 International Journal of Stem Cells