International Journal of Stem Cells : eISSN 2005-5447

Fig. 5.

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Fig. 5. Induction of p21 from the early stage severely blocked the emergence of CD43+ cells. KDR+ or GFP+KDR+ cells sorted from D0-induced or non-induced p21/hESC co-cultures at D2. About 5×103 cells were re-plated on irradiated AGM-S3 in 24-well plates or further hematopoietic culture, treated with or without DOX for 8 days, and then subjected to FACS analysis using a combination of CD34/KDR or CD34/CD43 antibodies. The results indicated that induction of p21 from D0 or D2 significantly blocked the appearance of CD43+ cells, but not CD34+ cells.
International Journal of Stem Cells 2020;13:202-11 https://doi.org/10.15283/ijsc20033
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