International Journal of Stem Cells : eISSN 2005-5447

Fig. 6.

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Fig. 6. Overexpression of p21 from D0 blocked the emergence of CD43+ cells in co-cultures with AGM-S3 cells. p21/hESC co-cultures were treated with DOX from D0, and then subjected to FACS analysis using combinations of anti-CD34/CD43 antibodies to compare non-induced co-cultures and the GFP+ fraction of co-cultures at D4, D6, and D8. Production of CD34+CD43− cells was not significantly influenced, whereas production of CD34−CD43+ and CD34+CD43+ dropped significantly following induction of p21, indicating that p21 has inhibitory effects on CD43+ cells but not CD34+ cells.
International Journal of Stem Cells 2020;13:202-11 https://doi.org/10.15283/ijsc20033
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