International Journal of Stem Cells : eISSN 2005-5447

Fig. 1.

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Fig. 1. HAECs influenced the phagocytosis of human blood granulocytes in cocultures. Isolated blood granulocytes were cocultured with hAECs at various ratio and time (as detailed in the text). After coculture, neutrophils and HAECs were separated by adherence. For phagocytosis assay, the neutrophils were stimulated and added by fluorescent 1.0 μm diameter beads and run on the flow cytometer. The uptake of fluorescent beads causes the neutrophils become fluorescent too; thus the percent of fluorescent granulocytes presented as phagocytosis percentage (A); unstimulated neutrophils were used as negative control (B).
International Journal of Stem Cells 2020;13:212-20 https://doi.org/10.15283/ijsc19155
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