International Journal of Stem Cells : eISSN 2005-5447

Fig. 3.

Download original image
Fig. 3. Knockdown of SOCS1 reverses the inhibition of plasma cell formation by MSCs. B cells were stimulated with LPS (A), LPS/IL4 (B), LPS/TGFβ (C) for 23 hours and then co-cultured by adding MSC/CTLsh or MSC/SOCS1sh at a ratio of 1:10. After two days of co-cultivation, B cells were harvested, labeled with anti-B220 and anti-CD138 antibodies and analyzed for plasma cell formation by flow cytometry. Data were representative of three independent experiments. *p<0.05. Note: CTLsh, MSC/CTLsh; SOCS1sh, MSC/SOCS1sh.
International Journal of Stem Cells 2020;13:237-45 https://doi.org/10.15283/ijsc20001
© 2020 International Journal of Stem Cells