International Journal of Stem Cells : eISSN 2005-5447

Fig. 5.

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Fig. 5. Multipotent differentiation potential analysis after 72 h treatment with UCMSCs-CM. (A) Osteogenic differentiation for 21 days, Scale bar=100 μm (magnification 100×), (B) adipogenic differentiation for 21 days, Scale bar=100 μm (magnification 100×), (C) chondrogenic differentiation for 21 days, Scale bar=50 μm (magnification 200×). The data are presented as the means±SD, n=3. *p<0.01, vs. D-NPSCs group. D-NPSCs exhibited few calcium deposition stained by Alizarin red S, whereas the cells from CM group displayed larger and more intensely stained mineralized nodules though it presented less intensely staining than UCMSCs. (A) There were no significant difference in Oil red O positive staining area between the CM group and D-NPSCs group, both appeared to form less fat drops than UCMSCs as shown in (B); Cells from CM group produced more intensely stained extracellular matrix than D-NPSCs group, showed similar intensity levels with UCMSCs group. (C) For osteogenic and chondrogenic differentiation, further quantitative analysis also revealed that the percentage of area stained positively was significantly lower in D-NPSCs group than that in both CM group and UCMSCs group.
International Journal of Stem Cells 2020;13:257-67 https://doi.org/10.15283/ijsc20027
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