International Journal of Stem Cells : eISSN 2005-5447

Fig. 2.

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Fig. 2. Long-term culture of hepatic organoids without passaging. (A) Scheme of long-term culture of the organoids. Matrigel-embedded organoids were maintained for four weeks without Matrigel renewal (upper). Organoids were divided at a 12 ratio and replated on fresh Matrigel weekly (lower). (B) Morphology of the organoids in the same field from week 1 to 4 without Matrigel renewal (upper) and with Matrigel renewal (lower). (C) mRNA expression levels of ALB, CK18, CK19, and AFP in organoids without Matrigel renewal and with Matrigel renewal weekly. Data are the mean±SEM (n=3) and analyzed by Student’s t-test, *p<0.05 and ***p<0.001.
International Journal of Stem Cells 2020;13:279-86 https://doi.org/10.15283/ijsc20060
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