International Journal of Stem Cells : eISSN 2005-5447

Fig. 3.

Download original image
Fig. 3. Long-term expansion of hepatic organoids by serial passaging. (A) Schematic diagram of long-term culture of organoids by passaging (upper). Representative bright field image of hepatic organoids after passaging at day 0, 1, 3 and 7 in the same field (lower). (B) Representative morphology of each passage of hepatic organoids at day 7. (C) Representative morphology of organoids a day after thawing (upper). Cell viability was determined by cell counting with Trypan blue staining before freezing and 12 hours after thawing (lower). Data are the mean±SEM (n=12). (D) Karyotype analysis of the organoids at passage 40 and 50. (E) mRNA expression levels of ALB and AFP at every 10 passages. Data are the mean±SEM (n=3) and analyzed by Student’s t-test, *p<0.05 and ***p<0.001.
International Journal of Stem Cells 2020;13:279-86
© 2020 International Journal of Stem Cells