International Journal of Stem Cells All Title Author Keywords Abstract   :: 선택 :: AND OR   International Journal of Stem Cells 2009 Nov; 2(2): 135~140 Adipose Tissue is A Better Source of Immature Non-Hematopoietic Cells than Bone Marrow Juri Fujimura1,2, Hidemitsu Sugihara3, Yoshitaka Fukunaga1, Hidenori Suzuki2, Rei Ogawa4,5 1Department of Pediatrics, Nippon Medical School, Tokyo, Japan, 2Department of Pharmacology, Nippon Medical School, Tokyo, Japan, 3Tumor Stem Cell Team, Biological Research Group, Pharmaceutical Research Laboratories, Research & Development Group, Nippon Kayaku Co. Ltd., Tokyo, Japan, 4Department of Plastic, Reconstructive and Aesthetic Surgery, Nippon Medical School, Tokyo, Japan, 5Division of Plastic Surgery, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA Adipose tissue (AT) is an alternative source of the adult stem cells that can also be harvested from bone marrow (BM). Cultured AT-derived stem cells (ASCs) have been well characterized by many groups. However, non-cultured ASCs remain to be characterized. Hoechst 33342 dye efflux is a characteristic that is common to stem cells, as well as chemotherapy-resistant cancer cells. Thus, we compared the Hoechst 33342-stained side population (SP) cells in murine adipose-tissue (AT-SP cells) to the SP cells from murine bone marrow (BM-SP cells). The AT-SP cells were detected much more frequently in the 22 AT samples that were tested (0.42∼6.00%, mean 2.57%) than the BM-SP cells were detected in the 6 BM samples (0.02∼0.36%, mean 0.12%). After Hoechst staining, SP cells were analyzed by fluorescence-activated cell sorting (FACS) and electron micrograms. FACS analysis revealed that the AT-SP cells were CD44-, CD45-, CD45R＋, Sca-1± and c-kit-, while the BM-SP cells were CD44-, CD45±, CD45R-, Sca-1＋ and c-kit＋. This indicates that the AT-SP cells differ phenotypically from the BM-SP cells. Electron microscopic analysis revealed that the AT-SP cells are small cells with a diameter of about 5 um. Some of the BM-SP cells had granules, similar to eosinophils or basophils, whereas the AT-SP cells had fewer organelles and a higher N/C ratio than the BM-SP cells. This suggests that the AT-SP cells are considerably more immature than the BM-SP cells. Thus, it appears that AT is a better source of immature non-hematopoietic cells than BM. International Journal of Stem Cells 2009 Nov; 2(2): 135~140 Keyword : Adipose-derived stem cells, Adipose stem cells, Mesenchymal stem cells, Adipose tissue, Sp cells, Bone marrow