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Characterization and Differentiation of Circulating Blood Mesenchymal Stem Cells and the Role of Phosphatidylinositol 3-Kinase in Modulating the Adhesion
International Journal of Stem Cells
Published online April 30, 2019;  
© 2019 Korean Society for Stem Cell Research.

Yoon-Kyung Park1, Seong-Joo Heo2, Jai-Young Koak2, Gang-Seok Park2, Tae-Jun Cho3, Seong-Kyun Kim2, Jaejin Cho3

1Dental Research Institute, Seoul National University, Brain Korea 21, Seoul, Korea
2Dental Research Institute and Prosthodontics, Seoul National University Dental Hospital, School of Dentistry, Seoul National University, Seoul, Korea
3Department of Dental Regenerative Biotechnology, School of Dentistry, Seoul National University, Seoul, Korea
Correspondence to: Jaejin Cho
Department of Dental Regenerative Biotechnology, School of Dentistry, Seoul National University, 28 Yongun-dong, Chongno-gu, Seoul 03080 Korea
Tel: +82-2-740-8666, Fax: +82-2-3676-8730, E-mail: jcho@snu.ac.kr
Co-Correspondence to Seong-Kyun Kim
Dental Research Institute and Prosthodontics, Seoul National University Dental Hospital, School of Dentistry, Seoul National University, 28 Yongun-dong, Chongno-gu, Seoul 03080, Korea
Tel: +82-2-2072-2660, Fax: +82-2-2072-3860, E-mail: ksy0617@snu.ac.kr
Received December 18, 2018; Revised January 23, 2019; Accepted February 26, 2019.
This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Bone marrow mesenchymal stem cells (BM MSCs) can differentiate into multi-lineage tissues. However, obtaining BM MSCs by aspiration is difficult and can be painful; therefore peripheral blood (PB) MSCs might provide an easier alternative for clinical applications. Here, we show that circulating PB MSCs proliferate as efficiently as BM MSCs in the presence of extracellular matrix (ECM) and that differentiation potential into osteoblast in vitro and in vivo. Both BM MSCs and PB MSCs developed into new bone when subcutaneously transplanted into immune-compromised mice using hydroxyapatite/tricalcium phosphate as a carrier. Furthermore, LY294002 and Wortmannin blocked mesenchymal stem cell attachment in a dose-dependent manner, suggesting a role of phosphatidylinositol 3-kinase in MSC attachment. Our data showed that the growth of PB MSCs could be regulated by interaction with the ECM and that these cells could differentiate into osteoblasts, suggesting their potential for clinical applications.
Keywords : Peripheral blood stem cell, Mobilization, Homing, Phosphatidylinositol 3-kinase, Cell attachment


February 2019, 12 (1)