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Induction of Nestin Early Expression as a Hallmark for Mesenchymal Stem Cells Expression of PDX-1 as a Pre-disposing Factor for Their Conversion into Insulin Producing Cells
International Journal of Stem Cells
Published online December 31, 2016;  
© 2016 Korean Society for Stem Cell Research.

Marisela Martinez-Gamboa1,2, Delia Elba Cruz-Vega2, Jorge Moreno-Cuevas2, Maria Teresa Gonzalez-Garza2

1Escuela De Ciencias De La Salud, Valle de las Palmas, Universidad Autónoma de Baja California, Tijuana, B.C, CP 22263, México,
2Cell Therapy Group, Escuela Nacional De Medicina, Tecnológico de Monterrey, Monterrey, CP 64710, NL, México
Correspondence to: Maria Teresa Gonzalez-Garza
Cell Therapy Group, Escuela Nacional de Medicina, Tecnológico de Monterrey, Monterrey, CP 64710, NL, México
Tel: +52-81-8888-2415, Fax: +52-81-8888-2141 E-mail: mtgonzalezgarza@itesm.mx
; Accepted November 8, 2016.
This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/ licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Diabetes constitutes a worldwide epidemic that affects all ethnic groups. Cell therapy is one of the best alternatives of treatment, by providing an effective way to regenerate insulin-producing cells lost during the course of the disease, but many issues remain to be solved. Several groups have been working in the development of a protocol capable of differentiating Mesenchymal Stem Cells (MSCs) into physiologically sound Insulin Producing Cells (IPCs). In order to obtain a simple, fast and direct method, we propose in this manuscript the induction of MSCs to express NESTIN in a short time period (2 h), proceeded by incubation in a low glucose induced medium (24 h) and lastly by incubation in a high glucose medium. Samples from cell cultures incubated in high glucose medium from 12 to 168 h were obtained to detect the expression of INSULIN-1, INSULIN -2, PDX-1 and GLUT-2 genes. Induced cells were exposed to a glucose challenge, in order to assess the production of insulin. This method allowed us to obtain cells expressing PDX-1, which resembles a progenitor insulin-producing cell.
Keywords : Mesenchymal stem cells, PDX-1, Insulin producing cells, Nestin


November 2016, 9 (2)