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The Effect of Different Routes of Injection of Bone Marrow Mesenchymal Stem Cells on Parotid Glands of Rats Receiving Cisplatin: A Comparative Study
International Journal of Stem Cells
Published online August 31, 2017;  
© 2017 Korean Society for Stem Cell Research.

Eman Hany1, Mohammed A. Sobh2, Mazen T. Abou ElKhier1, Heba M. ElSabaa1,3, Ahmed R. Zaher1

1Department of Oral Biology, Faculty of Dentistry, Mansoura University, Mansoura, Egypt,
2Urology and Nephrology Center, Mansoura University, Mansoura, Egypt,
3Department of Oral Biology, School of Dentistry, Badr University, Cairo, Egypt
Correspondence to: Eman Hany
Department of Oral Biology, Faculty of Dentistry, Mansoura University, Mansoura, Egypt
Tel: +02-01006177647, Fax: +20-50239733 E-mail: emy.hany11@gmail.com
; Accepted July 20, 2017.
This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Background and Objectives: Cisplatin is a powerful antitumor chemotherapeutic agent that is widely used in the treatment of many cancers but it has many side effects on many organs including salivary glands. Bone marrow is considered to be a rich environment that comprises many types of stem cells of which BMSCs (Bone marrow mesenchymal stem cells) are the most studied with potentiality to differentiate into many cell types. This study was conducted to evaluate the effect of different routes of injection of BMSCs on parotid glands of rats receiving cisplatin.
Methods and Results: Sprague-Dawley rats were divided into 3 groups: a negative control group receiving phosphate buffered saline, a positive control group receiving cisplatin, and an experimental group where rats received cisplatin and then received iron oxide-labeled BMSCs by either intravenous or intraparotid routes or both. Animals were sacrificed at periods of 3,6,10 and 15 days after cisplatin injection, then histological, ultrastructural and immunohistochemical studies were done. The experimental stem cell treated group showed better histological features and increased PCNA proliferation index when compared to the control. The systemic and combination groups showed better results than the local group. Iron oxide-labeled cells were detected with Prussian blue stain.
Conclusions: This study proved that BMSCs can improve cisplatin induced cytotoxicity in parotid glands. Systemic administration showed to have a better effect than local intraparotid administration and comparable effect to combined administration.
Keywords : Parotid gland, Chemotherapy, Bone marrow, Stem cells, Transmission electron microscopy, Immunohistochemistry


May 2017, 10 (1)