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Optimal Xeno-free Culture Condition for Clinical Grade Stem Cells from Human Exfoliated Deciduous Teeth
International Journal of Stem Cells
Published online February 28, 2018;  
© 2018 Korean Society for Stem Cell Research.

Nathaphon Tangjit1, Surachai Dechkunakorn1, Niwat Anuwongnukroh1, Anuson Khaneungthong1, Hathaitip Sritanaudomchai2

Departments of 1Orthodontics, 2Oral Biology, Faculty of Dentistry, Mahidol University, Bangkok, Thailand
Correspondence to: Hathaitip Sritanaudomchai
Department of Oral Biology, Faculty of Dentistry, Mahidol University, 6 Yothi Road, Rajthawee, Bangkok 10400, Thailand
Tel: +66-2-200-7849, Fax: +66-2-200-7848
; Accepted January 25, 2018.
This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Background and Objectives: Stem cells from human exfoliated deciduous teeth (SHED) are a promising clinical resource for various tissue defects, including lumbar spondylosis, neural compression, and cleft palate. Use of media containing animal-derived serum carries potential risk of infectious diseases and unwanted immunogenicity. To increase the potential utility of SHED for clinical application, SHED was adapted to xeno-free conditions. Methods: Define xeno-free culture media were compared with the conventional serum containing media in the culture of SHED. Cultured SHED in different media were further characterized through proliferative capacities, cellular phenotype, and differentiation potential. Results: Selected xeno-free media were capable of supporting the growth of SHED. MSCGM-CD Bulletkit medium greatly increased the number and proliferate capacity of colony-forming unit-fibroblast than SHED cultured in other media. In addition, the characteristic surface markers expression and multipotent differentiation potential of SHED in the MSCGM-CD Bulletkit medium were comparable to those observed with serum-containing medium. Conclusions: The xeno-free medium described herein has the potential to be further used for the safe expansion and to determine efficient way to produce clinical grade dental stem cells for therapeutic applications.
Keywords : Xeno-free media, Dental pulp stem cells, Clinical grade cells, Proliferation, Primary tooth, Osteogenesis

November 2017, 10 (2)