search for




 

MiR-9 Controls Chemotactic Activity of Cord Blood CD34 Cells by Repressing CXCR4 Expression
Int J Stem Cells 2018;11:187-195
Published online November 30, 2018;  
© 2018 Korean Society for Stem Cell Research.

Tae Won Ha1,*, Hyun Soo Kang1,*, Tae-Hee Kim2,*, Ji Hyun Kwon1, Hyun Kyu Kim1, Aeli Ryu3, Hyeji Jeon3, Jaeseok Han1, Hal E. Broxmeyer4, Yongsung Hwang1, Yun Kyung Lee1, Man Ryul Lee1

1Soonchunhyang Institute of Medi-bio Science (SIMS), Soon Chun Hyang University, Cheonan, Korea
2Department of Obstetrics and Gynecology, Soon Chun Hyang University College of Medicine, Bucheon, Korea
3Department of Obstetrics and Gynecology, Soon Chun Hyang University Cheonan Hospital, Cheonan, Korea
4Microbiology and Immunology, Indiana University School of Medicine, Indianapolis, USA
Correspondence to: Man Ryul Lee
Soonchunhyang Institute of Medi-bio Science, Soon Chun Hyang University, Cheonan 31151, Korea
Tel: +82-41-413-5013, Fax: +82-41-413-5006, E-mail: leeman@sch.ac.kr
Yun Kyung Lee
Soonchunhyang Institute of Medi-bio Science, Soon Chun Hyang University, Cheonan 31151, Korea
Tel: +82-41-413-5031, Fax: +82-41-413-5006, E-mail: yunklee@sch.ac.kr
Yongsung Hwang
Soonchunhyang Institute of Medi-bio Science, Soon Chun Hyang University, Cheonan 31151, Korea
Tel: +82-41-413-5017, Fax: +82-41-413-5006, E-mail: yshwang0428@sch.ac.kr
*These authors contributed equally to this work.
Received July 24, 2018; Revised August 30, 2018; Accepted September 7, 2018.
This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Improved approaches for promoting umbilical cord blood (CB) hematopoietic stem cell (HSC) homing are clinically important to enhance engraftment of CB-HSCs. Clinical transplantation of CB-HSCs is used to treat a wide range of disorders. However, an improved understanding of HSC chemotaxis is needed for facilitation of the engraftment process. We found that ectopic overexpression of miR-9 and antisense-miR-9 respectively down- and up-regulated C-X-C chemokine receptor type 4 (CXCR4) expression in CB-CD34 cells as well as in 293T and TF-1 cell lines. Since CXCR4 is a specific receptor for the stromal cell derived factor-1 (SDF-1) chemotactic factor, we investigated whether sense miR-9 and antisense miR-9 influenced CXCR4-mediated chemotactic mobility of primary CB CD34 cells and TF-1 cells. Ectopic overexpression of sense miR-9 and antisense miR-9 respectively down- and up-regulated SDF-1-mediated chemotactic cell mobility. To our knowledge, this study is the first to report that miR-9 may play a role in regulating CXCR4 expression and SDF-1-mediated chemotactic activity of CB CD34 cells.
Keywords : microRNA, CXCR4, Cord blood, Cell migration


November 2018, 11 (2)