International Journal of Stem Cells : eISSN 2005-5447

Fig. 1.

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Fig. 1. TGF-β isoforms induce trophoblast progenitor differentiation and Glut-1 transactivation. SM10 cells were treated for 72 hrs with (A) vehicle control, (B) 5 ng/ml TGF-β1, (C) 5 ng/ml TGF-β2, or (D) 2 ng/ml TGF-β3 to induce differentiation. (E) Glut-1 luciferase reporter activity in SM10s cells treated with vehicle control, TGF-β1, TGF-β2, or TGF-β3. Cells were transiently transfected with Glut1-lux and pRLSV40 using Metafectene. Twenty-four hours post-transfection, cells were treated with vehicle control, TGF-β1 (5 ng/ml), TGF-β2 (5 ng/ml), or TGF-β3 (2 ng/ml) for 24 or 72 hours. Luciferase activity was analyzed using the dual luciferase assay system. The Glut1-lux transactivation values were normalized to the constitutively active reporter, pRLSV40. Error bars represent standard error of the mean. (θ) indicates a significant increase in Glut1 luciferase transactivation compared to vehicle control at 24 hours and (*) indicates a significant increase from control at 72 hours. p<0.05.
International Journal of Stem Cells 2018;11:111-20
© 2018 International Journal of Stem Cells