International Journal of Stem Cells : eISSN 2005-5447

Fig. 2.

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Fig. 2. TGF-β induces SM10 cell differentiation and growth inhibition. SM10 cells were treated with (A) vehicle control, (B) TGF-β2 (5 ng/ml), (C) Activin A (5 ng/ml), or (D) Nodal (250 μg/ml) for 72 hrs to induce differentiation. (E) After 72 hours of treatment, cells were incubated with 1 μCi/ml 3H-Thymidine per well for 4 hours. Cells were then lysed and counted in scintillation fluid. Percent growth inhibition was determined as the amount of 3H-Thymidine incorporation compared to control. Percent growth inhibition is normalized to vehicle treated cells. Error bars represent standard error of the mean. *p<0.001.
International Journal of Stem Cells 2018;11:111-20
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