International Journal of Stem Cells : eISSN 2005-5447

Table. 1.

Table. 1.

Differences in cholangiocyte protocols

AuthorCell SourceChemicals usedFunctional assay used
Sampaziotis et al. (29)Human induced pluripotent stem cells20 ng/ml EGFRhodamine 123 assay, cholyl-lysyl-fluorescein assay, ALP staining, GGT activity.
Dianat et al. (31)Human pluripotent stem cells and HepaRG cells50 ng/ml GH, 25 ng/ml EGF, 10 ng/ml IL-6, 10 μM sodium taurocholate hydrate.Acetylcholine, somatostatin and ATP stimulation and cholyl-lysyl-fluorescein assay
Ogawa et al. (32)Human pluripotent stem cells co-cultured with OP9 stromal cells20 ng/ml HGF, 50 ng/ml EGF, 5 ng/ml TGFβRhodamine 123 asay and Forskolin-induced swelling assay
De Assuncao et al. (33)Human induced pluripotent stem cells100 μg/ml TGFβCalcium stimulation
Kido et al. (34)CPM+progenitor cells40 ng/ml R-spondin 1, 40 ng/ml WNT3aNone

Key: Epidermal growth factor (EGF), Human Growth Hormone (GH), Interleukin-6 (IL-6), Hepatocyte growth factor (HGF), Transforming growth factorβ (TGFβ).

International Journal of Stem Cells 2019;12:183-94 https://doi.org/10.15283/ijsc18055
© 2019 International Journal of Stem Cells