Fig. 4. hCdH-Chol expressed cholangiocyte markers seen through confocal microscopy. (a) Negative control hCdH stained with CK19 (green), CFTR (red). (b) CK19 (green), CFTR (red). (c) CK7 (green), AE2 (red). (d) CK7 (green), AE2 (red), and merged together showing a lack of expression of these genes. (d) hCdH-chol stained with CK7 (green) and AE2 (red) shows upregulation of these genes as seen through RT-qpcr. The nuclei were counterstained with Hoechst 33342 (blue). Scale bars, 50 μm.

International Journal of Stem Cells 2019;12:183-94 https://doi.org/10.15283/ijsc18055

© 2019 International Journal of Stem Cells