Fig. 1. MBP-FGF2-immobilized matrix can support the activation of SkMSCs. (A) Immunofluorescence staining images for the expression of myogenic markers, MyoD and myogenin (MYOG), in quiescent SkMSCs grown on Matrigel or MBP-FGF2 matrix for 48 h in growth medium. Cell nuclei were counterstained with DAPI. Scale bar, 50 μm. (B) Quantitation of MyoD- and MYOG-positive cells. Marker-positive cells are presented as the percentage of total cell number from triplicate samples. Error bars indicate standard error of the mean (SEM). NS, no statistical significance.