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Fig. 3. SkMSCs maintain robust myogenic potential on MBP-FGF2-mmobilized matrix. (A) Immunofluorescence staining images for MyHC expression in the SkMSCs cultured on Matrigel- or MBP-FGF2 (50 μg/mL)-coated surfaces at differentiation day 3. Cell nuclei were counterstained with DAPI. Scale bar, 50 μm. (B) To calculate differentiation indices after 3 days, MyHC-positive cell nuclei were counted and expressed as the percentage of total cell nuclei. (C) Fusion indices were shown as a distribution of MyHC-positive cells based on cell nucleus number; mono, 2~5, and more than 5 nuclei. Experiments were performed in triplicates. Error bars indicate SEM. NS, no statistical significance.
International Journal of Stem Cells 2019;12:360-6 https://doi.org/10.15283/ijsc18125
© 2019 International Journal of Stem Cells