International Journal of Stem Cells : eISSN 2005-5447

Fig. 3.

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Fig. 3. Effect of MUC1-C inhibition on STAT3 signaling and expression of pluripotency marker in mES cells. (A) The cells were serum starved overnight and then preincubated for 1 hr in the presence of CP1 or GO201 (10 μM). They were then stimulated with FBS (15%) and LIF for the indicated period and analyzed by western blotting. (B) The cells were treated with CP1 or GO201 at the indicated concentrations for three days and analyzed by western blotting. (C) The cells were treated with CP1 or GO201 at the indicated concentrations for 24 hr. Expression levels of MUC1 mRNA and protein were analyzed by RT-PCR (upper) and flow cytometry (lower). The histogram peak represents the expression of MUC1-C: Grey line, negative control; Black line, untreated control; Red line, CP1- or GO201-treated samples. (D) The cells were treated with CP1 or GO201 at the indicated concentrations for 24 hr. Expression levels of Oct4 mRNA and protein were analyzed by RT-PCR (upper) and western blotting (lower). Con, Control; CP, CP1; GO, GO201.
International Journal of Stem Cells 2021;14:180-90 https://doi.org/10.15283/ijsc20089
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