International Journal of Stem Cells : eISSN 2005-5447

Fig. 5.

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Fig. 5. MUC1-C inhibition increases ROS level in mES cells. (A) ROS level measured by flow cytometry using DCF-DA. The cells were treated with the indicated concentrations of GO201 or CP1 for 24 hr, incubated with 10 μM of DCF-DA for 30 min, and analyzed by flow cytometry. (B) The cells were treated with the indicated concentrations of GO201 or CP1 for 24 hr. The cell lysates were incubated with glutathione reductase and the GSH substrate, and the absorbance values were measured at a wavelength of 405 nm. (C) The cells were untreated or treated with 10 μM of NAC and were treated with the indicated concentrations of GO201 or CP1 for 24 hr. The cells were then incubated with 10 μM of DCF-DA for 30 min and analyzed by flow cytometry. Each bar is expressed as the mean±SD of three experiments. *p<0.05, **p<0.01, ***p<0.001 (vs CP1). (D) Images of mES cell colonies. The cells were untreated or treated with the indicated concentrations of NAC and were treated with 20 μM of GO201 or CP1 for 24 hr. Con, Control; CP, CP1; GO, GO201.
International Journal of Stem Cells 2021;14:180-90 https://doi.org/10.15283/ijsc20089
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