International Journal of Stem Cells : eISSN 2005-5447

Fig. 1.

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Fig. 1. The expression of genes implicated in specification and differentiation of ESCs into motor neurons. (a) The schematic representation of motor neuron differentiation used in this study. The detailed description of the procedure is presented in Materials and Methods. (b∼d) qRT-PCR analysis for the expression of stemness genes (Nanog and Oct4) (b), motor neuron differentiation markers (Olig2, Nkx6.1, Hb9 and Isl1) (c) and Shh signaling components (Shh, Ptch1, Gli1, Gli2, Gli3, Cdo, Boc and Gas1) (d) during specification and differentiation of mESCs into motor neuron. L32 was selected as a reference gene. Data represent means±SEM. *p<0.05, **p<0.01, ***p<0.001 (n=3, each).
International Journal of Stem Cells 2020;13:342-52
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