International Journal of Stem Cells : eISSN 2005-5447

Fig. 2.

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Fig. 2. Cdo deficiency altered gene expression in motor neuron specification of ES cells. (a) Scatter plot images of differentially expressed genes. Red and green colors indicate up- and down-regulated genes in Cdo−/− cells relative to Cdo+/+ cells, respectively (1.5-fold change, p<0.05). Black dots indicate genes with no significant change of RNA expression level (less than 1.5-fold change). (b) The bar charts indicate NES of the gene sets contributing to neuronal development in motor neuron specification-induced Cdo+/+ and Cdo−/− cells. (c) Heat map for the relative levels of the gene sets related to dorsal-, ventral-interneuron and spinal cord motor neuron in Cdo−/− cells compared to Cdo+/+ cells at MNS-3. Red=Up-regulated genes, Blue=Down-regulated genes. (d) qRT-PCR analysis for dorsal interneuron regulators (Pax3, Daam2 and Uncx) and motor neuron specific gene (Nkx6.1) in Cdo+/+ and Cdo−/− cells at MNS-3. Each value was normalized to the level of L-32, an endogenous control. Data represent means±SEM. *p<0.05, **p<0.01, ***p<0.001.
International Journal of Stem Cells 2020;13:342-52
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