International Journal of Stem Cells : eISSN 2005-5447

Fig. 3.

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Fig. 3. Cdo−/− ESCs displayed impaired motor neuronal specification. (a, b) qRT-PCR analysis for the levels of ventral and motor neuron specification markers (Pax6, Olig2, Nkx6.1, Hb9, Isl1 and Chx10) (a) and dorsal interneuron specification markers (Brn3a, Pax2, Lbx1 and Pax3) (b) in Cdo+/+ and Cdo−/− mESCs at MNS-5 and Elong stages. L32 was selected as a reference gene. Data represent means±SEM. *p<0.05, **p<0.01, ***p<0.001. (n=3, each) (c) Immunofluorescent staining for motor neuronal markers (Olig2 and Nkx6.1) in Cdo+/+ and Cdo−/− EBs at MNS-5. Nuclei were visualized by DAPI. Size bar=25μm. (d) Quantification of Olig2- and Nkx6.1-positive cells shown in panel c. Data represent means±SD. **p<0.01, ***p<0.001. (n=6) (e) Immunofluorescent staining for motor neuronal markers (Hb9 and Isl1) in Cdo+/+ and Cdo−/− cells at Elong stage. NF was also immunostained as a neuronal differentiation marker. Nuclei were visualized by DAPI. Size bar=10μm. (f) Quantification of Hb9- and Isl1-positive cells shown in panel e. Data represent mean±SD. ***p<0.001 (n=6).
International Journal of Stem Cells 2020;13:342-52 https://doi.org/10.15283/ijsc20037
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