International Journal of Stem Cells : eISSN 2005-5447

Fig. 1.

Download original image
Fig. 1. Profiling comparison of secretory proteins in FBS-MSC-CM and hPL-MSC-CM. (A) Analysis of proteome profiler human XL cytokines in FBS-MSC-CM and hPL-MSC-CM were determined in parallel. Reference spots are indicated by square boxes on the membrane blot. (B) Classification of secreted proteins detected in FBS-MSC-CM and hPL-MSC-CM by the relative analysis of pixel density using image J, we plotted the proteins detected in both MSC-CMs and in hPL-MSC-CM alone. (C) Secretory proteins detected in FBS-MSC-CM and hPL-MSC-CM. (D) Secretory proteins detected at a higher concentration in hPL-MSC-CM. (E) Secretory proteins detected in hPL-MSC-CM alone. (F) Concentrations of HGF, VEGF, and TGF-β1 in both MSC-CMs; HGF, VEGF, and TGF-β1 were analyzed quantitatively by ELISA in FBS-MSC-CM and hPL-MSC-CM. Data are represented as mean±SD. *<0.05, **<0.01, ***<0.001, ****<0.0001.
International Journal of Stem Cells 2021;14:94-102 https://doi.org/10.15283/ijsc20070
© 2020 International Journal of Stem Cells