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Fig. 2.

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Fig. 2. Cell density-dependent changes in senescence of MSCs in long-term cultures. (a) At passages 5, 10, and 15, senescence changes were observed in LD, MD, and HD using β-galactosidase staining. Scale bar: 500 μm. (b) Quantification of SA-β-gal activity in LD, MD, and HD. Senescence change was measured using the quantitative cellular senescence assay kit and expressed as relative fluorescence unit (RFU). (c) At passage 15, transcriptional changes in p15 and p16 genes, which are associated with senescence, and proliferating cell nuclear antigen (PCNA), a marker for cell proliferation, were analyzed using quantitative real-time PCR in LD, MD, and HD. (d) Changes in telomerase activities were measured using a Telomerase PCR ELISA Kit in LD, MD, and HD. Significance is *p<0.05, **p<0.01, ****p<0.0001.
International Journal of Stem Cells 2021;14:103-11 https://doi.org/10.15283/ijsc20078
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