International Journal of Stem Cells : eISSN 2005-5447

Fig. 4.

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Fig. 4. Cell density-dependent production of total ROS and DNA damage in long-term cultures. (a) Total ROS were detected using 2’, 7’-dichlorofluorescein diacetate (DCFDA) assay. MSCs were treated with 10 μM DCFDA solution and fluorescence was quantified using a fluorescence reader (excitation=485 nm and emission=535 nm). (b) Oxidative DNA damage was analyzed by measuring 8-OHdG produced in LD, MD, and HD. (c) MSC proliferation was compared among HD at passages 11, 13, and 15, with and without AA (Ascorbic acid, 25 μg/ml) treatment. FI and relative proliferation were calculated by cell counting. (d) At passage 15, ROS generation was analyzed in HD treated with or without AA by staining with DCFDA. (e) At passage 15, p15, p16, and PCNA expressions were analyzed in HD treated with or without AA by Quantitative PCR. (f) At passage 15, Oxidative DNA damage was analyzed in HD treated with or without AA by measuring 8-OHdG production. Significance is *p<0.05, **p<0.01, ****p<0.0001.
International Journal of Stem Cells 2021;14:103-11 https://doi.org/10.15283/ijsc20078
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