International Journal of Stem Cells : eISSN 2005-5447

Fig. 1.

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Fig. 1. Optimization of transfection in PD-MSCs. (A) Maps of GFP, lentiviral, and nonviral plasmid vectors. (B) GFP expression using 2 and 5 μg of PRL-1 plasmid using nonviral electroporation as well as the lentiviral system. Scale bar=100 μm. (C) Cell viability at 24 h after transfection. (D) mRNA expression of PRL-1 in PD-MSCsPRL-1. *p<0.05 versus GFP; **p<0.05 versus 2 μg; #p<0.05 versus lentiviral system. (E) Stemness markers (e.g., Oct4, Nanog, Sox2), TERT, and HLA-G expression in PD-MSCsPRL-1 were determined by RT-PCR. Data from each group are expressed as the mean±SD.
International Journal of Stem Cells 2021;14:112-8 https://doi.org/10.15283/ijsc20117
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