International Journal of Stem Cells : eISSN 2005-5447

Table. 1.

Table. 1.

Primer sequences using RT- and qRT-PCR

Genes Primer sequences Tm
Oct4 Forward 5'-AGTGAGAGGCAACCTGGAGA-3' 52
Reverse 5'-GTGAAGTGAGGGCTCCCATA-3'
Nanog Forward 5'-TTCTTGACTGGGACCTTGTC-3' 52
Reverse 5'-GCTTGCCTTGCTTTGAAGCA-3'
Sox2 Forward 5'-GGGCAGCGTGTACTTATCCT-3' 52
Reverse 5'-AGAACCCCAAGATGCACAAC-3'
TERT Forward 5'-GAGCTGACGTGGAAGATGAG-3' 55
Reverse 5'-CTTCAAGTGCTGTCTGATTCCAATG-3'
HLA-G Forward 5'-GCGGCTACTACAACCAGAGC-3' 58
Reverse 5'-GCACATGGCACGTGTATCTC-3'
β-actin Forward 5'-TCCTTCTGCATCCTGTCAGCA-3' 58
Reverse 5'-CAGGAGATGGCCACTGCCGCA-3'
Human Alu Forward 5'-GGAGGCTGAGGCAGGAGAA-3' 55
Reverse 5'-CGGAGTCTCGCTCTGTCGCCCA-3'
BGLAP Forward 5'-CACTCCTCGCCCTATTGGC 58
Reverse 5'-CCCTCCTGCTTGGACACAAAG-3'
COL1A1 Forward 5'- AGACATCCCACCAATCACCT-3' 60
Reverse 5'-CGTCATCGCACAACACCT-3'
Adipsin Forward 5'-GGTCACCCAAGCAACAAAGT-3' 60
Reverse 5'-CCTCCTGCGTTCAAGTCATC-3'
PPARG Forward 5'-TTGACCCAGAAAGCGATTCC-3' 60
Reverse 5'-AAAGTTGGTGGGCCAGAATG-3'
Albumin Forward 5'-TGAGTTTGCAGAAGTTTCCA-3' 60
Reverse 5'-CCTTTGCCTCAGCATAGTTT-3'
TAT Forward 5'-AACGATGTGGAGTTCACGG-3' 58
Reverse 5'-GACACATCCTCAGGAGAATGG-3'
PRL-1 Forward 5'-TACTGCTCCACCAAGAAGCC-3' 60
Reverse 5'-AGGTTTACCCCATCCAGGTC-3'
GAPDH Forward 5'-GCACCGTCAAGGCTGAGAAC-3' 60
Reverse 5'-GTGGTGAAGACGCCAGTGGA-3'
International Journal of Stem Cells 2021;14:112-8 https://doi.org/10.15283/ijsc20117
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