International Journal of Stem Cells : eISSN 2005-5447

Fig. 2.

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Fig. 2. Differentiation of hPSC into hepatobiliary organoids (HBOs). (A) Schematic presentation of the protocol for the generation of hPSC-derived HBOs. (B) Bright-field images of cells at key stages of HBO differentiation. The procedure steps and day numbers corresponding to each image are noted for reference. Scale bars, 150 μm. EM, expansion medium; EMM, endoderm and mesoderm medium; HBCM, hepato-bi-liary culture medium; HBDM, hepato-biliary differentiation medium; HBMM, hepato-biliary maturation medium; PM, progenitor medium.
International Journal of Stem Cells 2021;14:119-26 https://doi.org/10.15283/ijsc20152
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