International Journal of Stem Cells : eISSN 2005-5447

Fig. 3.

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Fig. 3. Functional properties of hPSC-derived HBOs. (A) Production of albumin and urea in day-35 HBOs. Cryopreserved adult hepatocytes (CAHs) were used as positive controls; hPSCs were used as negative control. *p<0.05; **p<0.01; n=3. (B) CYP3A4 induction in day-35 and day-80 HBOs. The HBOs were treated with 25 μM rifampin (RIF), followed by assessment of CYP3A4 activity using bioluminescent substrates. CAH were used as positive control. *p<0.05; **p<0.01; n=3. (C) The biliary structures in HBOs demonstrating characteristic ALP staining. Scale bars, 100 μm. (D) GGT activity in day-35 and day-85 HBOs. Human intrahepatic biliary epithelial cells (IBEC) was used as positive control; substrate only was used as negative control. *p<0.01; n=3.
International Journal of Stem Cells 2021;14:119-26 https://doi.org/10.15283/ijsc20152
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