International Journal of Stem Cells : eISSN 2005-5447

Fig. 1.

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Fig. 1. The metabolic profile of DMSCs. Basal OCR was measured before oligomycin treatment, and it was subtracted after rotenone/antimycin treatment. (A) ATP-linked OCR was calculated by subtracting the basal OCR from the amount of respiration left after oligomycin was added. Proton leakage was determined by subtracting the non-mitochondrial OCR from the amount of respiration left after oligomycin was added. Maximal respiration was calculated by subtracting the maximal OCR from the non-mitochondrial OCR. The reserve capacity was determined by subtracting the maximal OCR from the basal OCR. (B) O2 consumption in the DMSCs of the psoriasis and control groups. The rates of O2 (OCR) were first measured in DMSCs under basal conditions and then sequentially after the addition of oligomycin (1 µM), carbonylcyanidep-(trifluoromethoxy), phenylhydrazone (FCCP) (2 μM), and rotenone (0.5 μM). (C) Graphs show the ATP-linked OCR, proton leak OCR, maximal OCR, reserve capacity and non-mitochondrial OCR in the psoriasis and control groups. (D, E) Histograms showing the relative mRNA expression levels of GLUT1 and HK2. ns p>0.05, *p< 0.05, ***p<0.001.
International Journal of Stem Cells 2021;14:85-93 https://doi.org/10.15283/ijsc20073
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