International Journal of Stem Cells : eISSN 2005-5447

Fig. 1.

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Fig. 1. MiR-23b-3p-overexpressing BMSCs promoted the differentiation of BMSCs into TypeII pneumocytes and alleviated injuries in ALI lung tissues. (A, B, D, E, H) The expressions of miR-23b-3p, SPC, Oct4, Nanog and Occludin in the lung tissues of LPS-induced ALI mice were analyzed by qRT-PCR, after injection of BMSCs with no altered gene expression or BMSCs with miR-27a-3p overexpression or underexpression. (C, F, I) The expressions of SPC, Oct4, Nanog and Occludin in the lung tissues of LPS-induced ALI mice were analyzed by immunoblot, with GAPDH serving as a reference gene, after injection of BMSCs with no altered gene expression or BMSCs with miR-27a-3p overexpression or underexpression. (G) The ratio of LWW to BW of LPS-induced ALI mice was calculated, after injection of BMSCs with no altered gene expression or BMSCs with miR-27a-3p overexpression or underexpression. (J) Histopathological changes in the lung tissues of LPS-induced ALI mice were observed via hematoxylin-eosin staining, after injection of BMSCs with no altered gene expression or BMSCs with miR-27a-3p overexpression or underexpression (scale: 100 μm; magnification: ×100). (K∼P) The levels of IL-1β, IL-6, IL-4, IL-8, TNF-α and IL-10 in the bronchoalveolar lavage fluid of LPS-induced ALI mice were assessed by ELISA, after injection of BMSCs with no altered gene expression or BMSCs with miR-27a-3p overexpression or underexpression. #p<0.05; ##p<0.01; ***p or ###p<0.001; * vs. Control ; # vs. LPS+NC (LPS: lipopolysaccharide, SPC: surfactant protein C, NC: negative control, M: miR-23b-3p mimic, I: miR-23b-3p inhibitor, qRT-PCR: Quantitative reverse transcription polymerase chain reaction, ELISA: Enzyme-linked immunosorbent assay, ALI: acute lung injury, LWW/BW: lung wet weight/body weight, BMSCs: bone marrow-derived mesenchymal stem cells).
International Journal of Stem Cells 2021;14:229-39 https://doi.org/10.15283/ijsc20136
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