International Journal of Stem Cells : eISSN 2005-5447

Fig. 3.

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Fig. 3. FGF2 expression was inhibited by miR-23b-3p overexpressing BMSCs and it inhibited the differentiation of BMSCs into TypeII pneumocytes. (A, C) The expressions of FGF2 and SPC in the lung tissues of LPS-induced ALI mice were analyzed by qRT-PCR, after injection of BMSCs with no altered gene expression, BMSCs with overexpression of miR-27a-3p or FGF2 alone, or BMSCs with co-overexpression of miR-27a-3p and FGF2. (B, D) The expressions of FGF2 and SPC in the lung tissues of LPS-induced ALI mice were analyzed by immunoblot, with GAPDH serving as a reference gene, after injection of BMSCs with no altered gene expression, BMSCs with overexpression of miR-27a-3p or FGF2 alone, or BMSCs with co-overexpression of miR-27a-3p and FGF2. #p<0.05; **p or ##p<0.01; ***p or ###p or ^^^p<0.001; * vs. NC+FGF2-NC ; # vs. M+FGF2-NC; ^ vs. NC+FGF2 (LPS: lipopolysaccharide, SPC: surfactant protein C, FGF2: fibroblast growth factor 2, NC: negative control, M: miR-23b-3p mimic, qRT-PCR: Quantitative reverse transcription polymerase chain reaction, BMSCs: bone marrow-derived mesenchymal stem cells).
International Journal of Stem Cells 2021;14:229-39 https://doi.org/10.15283/ijsc20136
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