International Journal of Stem Cells : eISSN 2005-5447

Fig. 4.

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Fig. 4. FGF2 reversed the effect of the implantation of BMSCs overexpressing miR-23b-3p on ALI lung tissues. (A) The ratio of LWW to BW of LPS-induced ALI mice was calculated, after injection of BMSCs with no altered gene expression, BMSCs with overexpression of miR-27a-3p or FGF2 alone, or BMSCs with co-overexpression of miR-27a-3p and FGF2. (B) The expression of Occludin in the lung tissues was analyzed by qRT-PCR, after injection of BMSCs with no altered gene expression, BMSCs with overexpression of miR-27a-3p or FGF2 alone, or BMSCs with co-overexpression of miR-27a-3p and FGF2. (C) The expression of Occludin in the lung tissues of LPS-induced ALI mice was analyzed by immunoblot, with GAPDH serving as a reference gene, after injection of BMSCs with no altered gene expression, BMSCs with overexpression of miR-27a-3p or FGF2 alone, or BMSCs with co-overexpression of miR-27a-3p and FGF2. (D) Histopathological changes in the lung tissues of LPS-induced ALI mice were observed via hematoxylin-eosin staining, after injection of BMSCs with no altered gene expression, BMSCs with overexpression of miR-27a-3p or FGF2 alone, or BMSCs with co-overexpression of miR-27a-3p and FGF2 (scale: 100 μm; magnification: ×100). (E∼J) The levels of IL-1β, IL-6, IL-4, IL-8, TNF-α and IL-10 in the bronchoalveolar lavage fluid of LPS-induced ALI mice were assessed by ELISA, after injection of BMSCs with no altered gene expression, BMSCs with overexpression of miR-27a-3p or FGF2 alone, or BMSCs with co-overexpression of miR-27a-3p and FGF2. *p or #p<0.05; **p or ##p or ^^p<0.01; ***p or ###p or ^^^p<0.001; * vs. NC+FGF2-NC ; # vs. mimic+FGF2-NC; ^ vs. NC+FGF2 (LPS: lipopolysaccharide, FGF2: fibroblast growth factor 2, NC: negative control, M: miR-23b-3p mimic, qRT-PCR: Quantitative reverse transcription polymerase chain reaction, ELISA: Enzyme-linked immunosorbent assay, ALI: acute lung injury, LWW/BW: lung wet weight/body weight, BMSCs: bone marrow-derived mesenchymal stem cells).
International Journal of Stem Cells 2021;14:229-39 https://doi.org/10.15283/ijsc20136
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