International Journal of Stem Cells : eISSN 2005-5447

Table. 1.

Table. 1.

Organoids’ cultural system for SARS-CoV-2 research

Organoids formation Cytokines Markers expression Period/meaning Reference
Choroid plexus organoid

1. Y-27632: 0.5 um

2. LDN-193189: 5 um

3. SB-431542: 1 um

4. IWP-2: 3 um

5. CHIR99021: 200 ng/ml

6. BMP4: 10 ng/ml

7. BDNF: 130 ng/ml

8. GDNF: 130 ng/m

.ACE2

.TMPRSS2

.OTX2

.FOXG1

.LMX1

.TTR

.AQP1

.OTX2

3∼7 week Jacob et al.
Ocular organoid

1. FGF2: 5 ng/ml

2. KGF: 20 ng/ml

3. Y-27632: 10 μM

*(Noggin, LDN-193189, SB-431542, BMP4 are added at the first four days, but the concentration is unknown)

.ACE2

.TMPRSS2

.Zone 3

.E-cadherin

.Pax6

.ZO-1

.KRT5

.KRT8

.KRT19

.KRT13

.KRT15

.KRT19

.AQP3

8∼12 week Hayashi et al.
Lung organoid

1. Activin A: 100 ng/ml

2. SB431542: 10 μM

3. CHIR99021: 5 μM

4. FGF7: 10 ng/ml

5. FGF10: 10 ng/ml

6. CHIR99021: 3 μM

7. EGF: 10 ng/ml

8. VEGF: 10 ng/ml

9. PIGF: 10 ng/ml

10. cAMP: 100 μM

11. IBMX: 100 μM

12. BMP4: 10 ng/ml

.ACE2

.TMPRSS2

.SOX2

.FOXA2

.NKX2

.SPB

.SPC

About 7 week Han et al.
Human bronchial organoids

1. FGF2: 5 ng/ml

2. FGF7: 20 ng/ml

3. FGF10: 100 ng/ml

4. Noggin: 100 ng/ml

5. R-spondin1: 300 ng/ml

6. Y-27632: 10 μM

7. SB202190: 10 μM

8. A83-01: 10 μM

.ACE2

.TMPRSS2

.NGFR

.PROM1

.TUBA1A

.MCIDAS

.MUC20

.MUC5B

.SCGB1A1

.KLF5

.α-tubulin

.CC10

.mucin 5AC

.KRT5

About 1 week Tan et al.
Heart organoid

1. BMP-4: 20 ng/mL

2. activin A: 6 ng/mL

3. IWP-4: 5 mM

4. IWR-1: 10 mM

.ACE2

.TMPRSS2

.MLC2v

.CD90

.CD31

.WT-1

.Ki-67

.Around 2 week Mills RJ et al.
Intestinal organoid (human and bat)

1. A8301: 500 nM

2. SB202190: 10 μM

3. hEGF: 50 ng ml−1

4. Y-27632: 10 μM

5. hGastrin I: 10 nM

.ACE2

.TMPRSS2

.CTSL

.SMOC2

.CDCA7

.OLFM4

.ASCL2

.AXIN2

.Lgr5

.4∼12 week

.The first bat intestinal organoid

Zhou et al.
Liver ductal organoid

1. EGF: 50 ng/ml

2. FGF10: 100 ng/ml

3. HGF: 25 ng/m

4. Nicotinamide: 10 mM

5. A83-01: 5 uM

6. FSK: 10 uM

7. Noggin: 25 ng/ml

8. Y27632: 10 uM

.ACE2

.TMPRSS2

.EPCAM

.keratin 19

.1 week

.The first infection model reported of SARS-CoV-2 human organoid

Zhao et al.
Kidney organoid

1. CHIR99021: 8 mM

2. FGF9: 200 ng/ml

3. Heparin: 1 mg/ml

4. Activin A: 10 ng/ml

.ACE2

.TMPRSS2

.SLC3A1

.SLC27A2

.PODXL

.NPHS1

.NPHS2

About 3 weeks Monteil et al.
Capillary organoid

1. CHIR99021: 12 μm

2. SB431542: 10 um

3. BMP4: 30 ng/ml

4. VEGF-A: 2 μm

5. FGF-2: 100 ng/ml

6. Y-27632: 50 μm

7. Forskolin: 2 μm

.ACE2

.TMPRSS2

.CD31

.UEA-I

.von-Willebrand

.VE-PTP,

.NG2

.SMA

.calponin 1

.ICAM-1

>2 months Wimmer et al.
International Journal of Stem Cells 2021;14:138-49 https://doi.org/10.15283/ijsc20169
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